055 Whole-transcriptome Sequencing-Based Profiling of the Cutaneous Virome in Patients with Secondary Immunodeficiency

The majority of viral infections can be self-limited in normal populations, with no requirement for medical intervention. However, the same viruses cause severe diseases patients compromised immunity due to single-gene diseases, AIDS, hematologic malignancies, or those receiving immunosuppressive drugs following organ transplant. Occasionally, these immunocompromised harbor more than one infectious agent, making it necessary perform several concomitant diagnostic tests. prototype such conditions is group associated human papillomaviruses (HPV), a family over 400 genetically distinct types. We have developed novel whole-transcriptome sequencing (RNA-Seq) based pipeline that allows virome profiling, quantitation, and expression pattern analysis 926 by RNA isolated from single lesional skin biopsy. applied this six lesions immune deficiency secondary HIV, HTLV1, chronic lymphocytic leukemia, postrenal transplant immunosuppression. In five cases, definitive HPV were identified, some cases caused multiple addition HPV, co-infection other (MCP, CMV, EBV) was detected lesions. case, identification HTLV1 adult T-cell leukemia/lymphoma. These findings attest power RNA-Seq profiling cutaneous context immunity.